Twist1 Recombinant Rabbit Monoclonal Antibody [PSH11-98] (HA723411)
Catalog# HA723411
Rabbit Monoclonal to Twist1
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Twist1 on different lysates with Rabbit anti-Twist1 antibody (HA723411) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: A375 cell lysate
Lane 3: PC-3M cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: E14.0 mouse embryonic brain tissue lysate
Lane 8: Rat embryo tissue lysate
Lane 9: LLC cell lysate
Lane 10: Mouse embryo tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 24 kDa
Exposure time: Lane 1-8: 1 minute; Lane 9-10: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate
Lane 2: A375 cell lysate
Lane 3: PC-3M cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: E14.0 mouse embryonic brain tissue lysate
Lane 8: Rat embryo tissue lysate
Lane 9: LLC cell lysate
Lane 10: Mouse embryo tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 24 kDa
Exposure time: Lane 1-8: 1 minute; Lane 9-10: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.