TUB Recombinant Rabbit Monoclonal Antibody [JE58-75] (HA720035)
Catalog# HA720035
Rabbit Monoclonal to TUB
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WB
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Human
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Rat
Lane 1: HepG2 cell lysate
Lane 2: L6 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 65 kDa
Exposure time: Lane 1: 42 seconds; Lane 2: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720035) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
TUB Recombinant Rabbit Monoclonal Antibody [JE58-75] (HA720035)
Immunogen
Synthetic peptide within human tub aa 1-50/506.
Host
Rabbit
Positive Control
HepG2 cell lysate, L6 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE58-75
RRID
APPLICATION DILUTION
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WB
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1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 56 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TUB
SYNONYMS
F10B5.4 antibody;rd5 antibody;TUB 1 antibody;TUB antibody;TUB_HUMAN antibody;Tubby homologue antibody;Tubby protein homolog 1 antibody;Tubby protein homolog antibody
SUBCELLULAR LOCATION
Secreted, Cell membrane, Nucleus, Cytoplasm.
FUNCTION
This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight (By similarity). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages.
CITATIONS
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Huang Panpan,et al
Regulation of TRI5 expression and deoxynivalenol biosynthesis by a long non-coding RNA in Fusarium graminearum
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WB
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Wheat
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Citation
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