Western blot analysis of TRPV6 on different lysates with Rabbit anti-TRPV6 antibody (HA500333) at 1/500 dilution.
Lane 1: SW480 cell lysate
Lane 2: SK-Br-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 42 kDa (unglycosylated monomeric form of TRPV6)
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500333) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Rabbit polyclonal primary
TRPV6 Rabbit Polyclonal Antibody (HA500333)
Synthetic peptide corresponding to c terminal of human trpv6 .
SW480 cell lysate, SK-Br-3 cell lysate, HepG2, HT-29, rat testis tissue, human kidney tissue, mouse colon tissue, human placenta tissue.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
ABP/ZF antibody;ABPZF antibody;Alu binding protein with zinc finger domain antibody;Calcium channel CaT1 antibody;Calcium transport protein 1 antibody;CaT L antibody;CaT Like antibody;CaT-L antibody;CaT-like antibody;CaT1 antibody;ecac antibody;ECaC2 antibody;Epithelial apical membrane calcium transporter/channel CaT1 antibody;Epithelial calcium channel 2 antibody;Epithelial calcium channel antibody;HSA277909 antibody;LP6728 antibody;MGC162545 antibody;NUDC antibody;Transient receptor potential cation channel subfamily V member 6 antibody;TrpV6 antibody;TRPV6_HUMAN antibody;ZFAB antibody
Cell membrane, Membrane
Calcium selective cation channel that mediates Ca2+ uptake in various tissues, including the intestine . Important for normal Ca2+ ion homeostasis in the body, including bone and skin (By similarity). The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification . Inactivation includes both a rapid Ca2+-dependent and a slower Ca2+-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg2+ in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.