Tropomyosin 2 Recombinant Rabbit Monoclonal Antibody [PSH02-63] (HA721841)
Catalog# HA721841
Rabbit Monoclonal to Tropomyosin 2
Tested for
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WB
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IHC-P
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IF-Tissue
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IF-Cell
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of Tropomyosin 2 on different lysates with Rabbit anti-Tropomyosin 2 antibody (HA721841) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: U-2 OS cell lysate
Lane 2: Saos-2 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: Mouse skeletal muscle tissue lysate
Lane 6: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 37 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721841) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: U-2 OS cell lysate
Lane 2: Saos-2 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: Mouse skeletal muscle tissue lysate
Lane 6: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 37 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721841) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.