PRODUCT CODE: ET7108-74

Tropomyosin 1 Recombinant Rabbit Monoclonal Antibody [JG38-66] (ET7108-74)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Tropomyosin 1 on rat heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Tropomyosin 1 on rat heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Tropomyosin 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-74, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Western blot analysis of Tropomyosin 1 on rat heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Tropomyosin 1 Recombinant Rabbit Monoclonal Antibody [JG38-66] (ET7108-74)

Immunogen

Recombinant protein within human tropomyosin 1 aa 100-300.

Host

Rabbit

Positive Control

Rat heart tissue, rat smooth muscle tissue, human heart tissue, mouse skeletal muscle tissue, MCF-7.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG38-66

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

33 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tropomyosin 1

SYNONYMS

AA986836 antibody; AI854628 antibody; Alpha tropomyosin antibody; alpha-TM antibody; Alpha-tropomyosin antibody; C15orf13 antibody; cardiomyopathy, hypertrophic 3 antibody; CMD1Y antibody; CMH3 antibody; HTM alpha antibody; HTM-alpha antibody; OTTHUMP00000163688 antibody; sarcomeric tropomyosin kappa antibody; TM2 antibody; Tmpa antibody; TMSA antibody; Tpm-1 antibody; TPM1 antibody; TPM1_HUMAN antibody; tropomyosin 1 (alpha) antibody; tropomyosin 1 (alpha) isoform 1 antibody; tropomyosin 1 (alpha) isoform 2 antibody; tropomyosin 1 (alpha) isoform 3 antibody; tropomyosin 1 (alpha) isoform 4 antibody; tropomyosin 1 (alpha) isoform 5 antibody; tropomyosin 1 (alpha) isoform 6 antibody; tropomyosin 1 (alpha) isoform 7 antibody; Tropomyosin 1 antibody; Tropomyosin alpha 1 chain antibody; Tropomyosin alpha-1 chain antibody; Tropomyosin, skeletal muscle alpha antibody; Tropomyosin-1 antibody

SEQUENCE SIMILARITIES

Belongs to the tropomyosin family.

TISSUE SPECIFICITY

Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells.

SUBCELLULAR LOCATION

Cytoskeleton.

FUNCTION

Tropomyosins are a group of structural proteins. Tropomyosins are present in virtually all eukaryotic cells, both muscle and non-muscle, where they bind Actin filaments and function to modulate Actin-Myosin interaction and stabilize Actin filament structure. Tropomyosin α is encoded by the TPM1 gene, which maps to human chromosome 15q22.1 and undergoes alternative splicing to generate at least four isoforms, including skeletal muscle (isoform 1), smooth muscle (isoform 2), fibroblast/TM3 (isoform 3) and isoform 4. Tropomyosin β is encoded by the TPM2 gene, which maps to human chromosome 9p13.3 and undergoes alternative splicing to generate three isoforms, including skeletal muscle (isoform 1), non-muscle/fibroblast TM36/epithelial TMe1 (isoform 2) and non-muscle (isoform 3). Troponin I binds Tropomyosin at a specific region and the association of Tropomyosin-Troponin with Actin filaments may increase the rigidity of Actin filaments. Tropomyosin also interacts with Caldesmon to regulate smooth muscle contraction.