PRODUCT CODE: ET1609-16

Tri-Methyl-Histone H4 (K20) Recombinant Rabbit Monoclonal Antibody [ST044-07] (ET1609-16)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H4 (tri methyl K20) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H4 (tri methyl K20) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H4 (tri methyl K20) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H4 (tri methyl K20) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H4 (tri methyl K20) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H4 (tri methyl K20) antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H4 (tri methyl K20) antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Tri-Methyl-Histone H4 (K20) Recombinant Rabbit Monoclonal Antibody [ST044-07] (ET1609-16)

Immunogen

Recombinant protein

Host

Rabbit

Modification

Tri-Methyl

Modification Site

K20

Positive Control

Human liver tissue, human kidney tissue, mouse liver tissue, mouse colon tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST044-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Tri-Methyl-Histone H4 (K20)

SYNONYMS

Histone gene cluster 1, H4A antibody; Histone gene cluster 2, H4 antibody; dJ160A22.1 antibody; dJ160A22.2 antibody; dJ221C16.1 antibody; dJ221C16.9 antibody; FO108 antibody; H4 antibody; H4 histone family, member A antibody; H4 histone family, member B antibody; H4 histone family, member C antibody; H4 histone family, member D antibody; H4 histone family, member E antibody; H4 histone family, member G antibody; H4 histone family, member H antibody; H4 histone family, member I antibody; H4 histone family, member J antibody; H4 histone family, member K antibody; H4 histone family, member M antibody; H4 histone family, member N antibody; H4 histone, family 2 antibody; H4/A antibody; H4/B antibody; H4/C antibody; H4/D antibody; H4/E antibody; H4/G antibody; H4/H antibody; H4/I antibody; H4/J antibody; H4/K antibody; H4/M antibody; H4/N antibody; H4/O antibody; H4/p antibody; H4_HUMAN antibody; H4F2 antibody; H4F2iii antibody; H4F2iv antibody; H4FA antibody; H4FB antibody; H4FC antibody; H4FD antibody; H4FE antibody; H4FG antibody; H4FH antibody; H4FI antibody; H4FJ antibody; H4FK antibody; HIST1 cluster, H4A antibody; HIST1 cluster, H4B antibody; HIST1 cluster, H4D antibody; HIST2H4 antibody; Hist4 cluster, H4 antibody; Hist4h4 antibody; histone 1, H4a antibody; histone 1, H4c antibody; histone 1, H4d antibody; histone 1, H4f antibody; histone 1, H4h antibody; histone 1, H4i antibody; histone 1, H4j antibody; histone 1, H4k antibody; histone 1, H4l antibody; histone 2, H4a antibody; histone 2, H4b antibody; Histone 4 family, member M antibody; histone 4, H4 antibody; histone cluster 1, H4 antibody; histone cluster 1, H4a antibody; histone cluster 1, H4b antibody; histone cluster 1, H4c antibody; histone cluster 1, H4d antibody; histone cluster 1, H4e antibody; histone cluster 1, H4f antibody; histone cluster 1, H4h antibody; histone cluster 1, H4i antibody; histone cluster 1, H4j antibody; histone cluster 1, H4k antibody; histone cluster 1, H4l antibody; histone cluster 2, H4a antibody; histone cluster 2, H4b antibody; histone cluster 4, H4 antibody; Histone family, member A antibody; Histone family, member B antibody; Histone family, member D antibody; Histone family, member H antibody; Histone family, member I antibody; Histone family, member L antibody; Histone gene cluster 1, H4 antibody; Histone gene cluster 1, H4D antibody; Histone gene cluster 1, H4E antibody; Histone gene cluster 1, H4K antibody; Histone gene cluster 4, H4 antibody; Histone gene cluster 4, H4 histone antibody; Histone H4 antibody; histone IV, family 2 antibody

SEQUENCE SIMILARITIES

Belongs to the histone H4 family.

POST-TRANSLATIONAL MODIFICATION

Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.; Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.; Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.; Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Dimethylation and trimethylation is performed by KMT5B and KMT5C and induces gene silencing (By similarity).; Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.; Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).; Sumoylated, which is associated with transcriptional repression.; Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.; Butyrylation of histones marks active promoters and competes with histone acetylation.; Glutarylation at Lys-92 (H4K91glu) destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes.

SUBCELLULAR LOCATION

Nucleus, Chromosome.

FUNCTION

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to trimethylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.