Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
TREM2 Rabbit Polyclonal Antibody (ER2001-05)
Immunogen
Synthetic peptide within human trem2 isoform 2 aa 150-190.
Host
Rabbit
Positive Control
U937 cell lysates, human liver tissue, human liver carcinoma tissue, human colon tissue, human kidney tissue, THP-1.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
25 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB:1:500-1:1,000
-
IHC-P:1:50-1:200
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
TREM2
SYNONYMS
TREM 2 antibody; TREM-2 antibody; TREM2 antibody; TREM2_HUMAN antibody; TREM2a antibody; TREM2b antibody; TREM2c antibody; Trggering receptor expressed on myeloid cells 2 antibody; Trggering receptor expressed on myeloid cells 2a antibody; Triggering receptor expressed on monocytes 2 antibody; Triggering receptor expressed on myeloid cells 2 antibody
SUBCELLULAR LOCATION
Cell membrane, Secreted.
FUNCTION
Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding. Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia. Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokinesand the anti-inflammatory cytokine ARG1. Acts as a receptor for lipoprotein particles and HDL and for apolipoproteins and CLU and enhances their uptake in microglia. Binds phospholipids such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin. Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade. Required for microglial phagocytosis of apoptotic neurons. Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain. Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide. It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB. During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling. Plays a role in microglial MTOR activation and metabolism. Regulates age-related changes in microglial numbers. Triggers activation of the immune responses in macrophages and dendritic cells. Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages.