PRODUCT CODE: 0903-8

Transmembrane protein 132A Rabbit Polyclonal Antibody (0903-8)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Transmembrane protein 132A on mouse brain lysate lysate using anti-Transmembrane protein 132A antibody at 1/1,000 dilution.
  • Western blot analysis of Transmembrane protein 132A on mouse brain lysate lysate using anti-Transmembrane protein 132A antibody at 1/1,000 dilution.
  • ICC staining Transmembrane protein 132A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Transmembrane protein 132A in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Transmembrane protein 132A antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with Transmembrane protein 132A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Transmembrane protein 132A on mouse brain lysate lysate using anti-Transmembrane protein 132A antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Transmembrane protein 132A Rabbit Polyclonal Antibody (0903-8)

Immunogen

Synthetic peptide within human transmembrane protein 132a aa 204-253 / 1,023.

Host

Rabbit

Positive Control

Mouse brain tissue, Hela, SHG-44, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Transmembrane protein 132A

SYNONYMS

GBP antibody; HSPA5-binding protein 1 antibody; HSPA5BP1 antibody; KIAA1583 antibody; T132A_HUMAN antibody; Tmem132a antibody; Transmembrane protein 132A antibody

SEQUENCE SIMILARITIES

Belongs to the TMEM132 family.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Golgi apparatus. Membrane.

FUNCTION

TMEM132A is a member of TMEM132 family. Total of five members, namely TMEM132 A, B, C, D, E, were found. TMEM132A, also known as HSPA5-binding protein 1, may play a role in embryonic and postnatal development of the brain and regulate cAMP-induced GFAP gene expression via STAT3 phosphorylation.