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PRODUCT CODE: ER2001-53

Transferrin Rabbit Polyclonal Antibody (ER2001-53)

Applications

  • WB

  • IHC

  • FC

REACTIVITY

  • Human

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Western blot analysis of Transferrin on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Transferrin on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-53, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Transferrin was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-53, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Transferrin on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Transferrin Rabbit Polyclonal Antibody (ER2001-53)

Immunogen

Synthetic peptide within human transferrin aa 500-550.

Host

Rabbit

Positive Control

Human liver tissue, HepG2 cell lysates, HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

77 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:5,000

  • IHC

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P02787

PROTEIN NAME

Transferrin

SYNONYMS

Apotransferrin antibody; Beta 1 metal binding globulin antibody; Beta-1 metal-binding globulin antibody; DKFZp781D0156 antibody; PRO1400 antibody; PRO1557 antibody; PRO2086 antibody; Serotransferrin antibody; Serotransferrin precursor antibody; Siderophilin antibody; TF antibody; TFQTL1 antibody; Transferin antibody; Transferrin antibody; TRFE_HUMAN antibody

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.