TPH2 Recombinant Antibody [PSH04-89] - Rat IgG1 (Chimeric) - BSA and Azide free (HA610324)
Catalog# HA610324
to TPH2
Tested for
-
WB
-
IHC-P
Reacts with
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of TPH2 on different lysates with Rat anti-TPH2 antibody (HA610324) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (negative) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: Rat kidney tissue lysate (negative) (20 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610324) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TPH2 on different lysates with Rat anti-TPH2 antibody (HA610324) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (negative) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: Rat kidney tissue lysate (negative) (20 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610324) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.