PRODUCT CODE: ET7108-80

TNPO3 Recombinant Rabbit Monoclonal Antibody [JG38-77] (ET7108-80)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of TNPO3 on K562 cell (1), HL-60 cell (2) and mouse pancreas tissue (3) lysate using anti-TNPO3 antibody at 1/1,000 dilution.
  • Western blot analysis of TNPO3 on K562 cell (1), HL-60 cell (2) and mouse pancreas tissue (3) lysate using anti-TNPO3 antibody at 1/1,000 dilution.
  • ICC staining TNPO3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TNPO3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TNPO3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.
Western blot analysis of TNPO3 on K562 cell (1), HL-60 cell (2) and mouse pancreas tissue (3) lysate using anti-TNPO3 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

TNPO3 Recombinant Rabbit Monoclonal Antibody [JG38-77] (ET7108-80)

Immunogen

Recombinant protein within human tnpo3 aa 800-950.

Host

Rabbit

Positive Control

HL-60, mouse pancreas tissue lysate, A549, SH-SY-5Y, SiHa, human liver cancer tissue, human small intestine tissue, mouse lung tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG38-77

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

104 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

TNPO3

SYNONYMS

TNPO 3 antibody; Imp12 antibody; Importin 12 antibody; Importin-12 antibody; IPO12 antibody; MTR10A antibody; TNPO3 antibody; TNPO3_HUMAN antibody; Transportin 3 antibody; Transportin SR antibody; Transportin-3 antibody; Transportin-SR antibody; TRN SR antibody; TRN-SR antibody; TRN-SR2 antibody; TRNSR antibody

TISSUE SPECIFICITY

Expressed in skeletal muscle.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

The Importin complex consists of Importin-α and Importin-β proteins which assist in transport of arginine- or serine-rich (SR) proteins across the nucleus. Importin-12, also known as Transportin-3 or Transportin-SR, is a member of the Importin-β family and functions as a nuclear transport receptor for serine/arginine-rich proteins. Through recognition of phosphorylated RS domains, Importin-12 mediates the nuclear import of several SR proteins, such as splicing factors SFRS1 and SFRS2. By regulating the nucleocytoplasmic transport of these and other SR mRNA splicing factors, Importin-12 controls their access to mRNA and, therefore, acts a transcriptional regulator.