TNFRSF14 Recombinant Rabbit Monoclonal Antibody [PSH06-02] (HA722501)
Catalog# HA722501
Rabbit Monoclonal to TNFRSF14
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT),Relative expression (RE)
Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody (HA722501) at 1/1,000 dilution.
Lane 1: SK-MEL-28 cell lysate
Lane 2: SK-MEL-28 cell lysate treated with deglycosylation
Lane 3: A549 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 40-50 kDa
Exposure time: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody (HA722501) at 1/1,000 dilution.
Lane 1: SK-MEL-28 cell lysate
Lane 2: SK-MEL-28 cell lysate treated with deglycosylation
Lane 3: A549 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 40-50 kDa
Exposure time: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.