PRODUCT CODE: ER1706-32

TNFAIP3 Rabbit Polyclonal Antibody (ER1706-32)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of TNFAIP3 on Jurkat (1) and Daudi (2) cell lysate using anti-TNFAIP3 antibody at 1/100 dilution.
  • Western blot analysis of TNFAIP3 on Jurkat (1) and Daudi (2) cell lysate using anti-TNFAIP3 antibody at 1/100 dilution.
  • ICC staining TNFAIP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TNFAIP3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with TNFAIP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of TNFAIP3 on Jurkat (1) and Daudi (2) cell lysate using anti-TNFAIP3 antibody at 1/100 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TNFAIP3 Rabbit Polyclonal Antibody (ER1706-32)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, Daudi, A431, LOVO, rat lung tissue, human liver cancer tissue, human kidney tissue, mouse testis tissue, HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

90 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

TNFAIP3

SYNONYMS

A20 antibody; AISBL antibody; MGC104522 antibody; MGC138687 antibody; MGC138688 antibody; OTU domain containing protein 7C antibody; OTU domain-containing protein 7C antibody; OTUD7C antibody; Putative DNA binding protein A20 antibody; Putative DNA-binding protein A20 antibody; TNAP3_HUMAN antibody; TNF alpha-induced protein 3 antibody; TNFA1P2 antibody; TNFAIP 3 antibody; TNFAIP3 (A20) antibody; TNFAIP3 antibody; Tumor necrosis factor alpha induced protein 3 antibody; Tumor necrosis factor alpha-induced protein 3 antibody; Tumor necrosis factor induced protein 3 antibody; Tumor necrosis factor inducible protein A20 antibody; tumor necrosis factor, alpha-induced protein 3 antibody; Zinc finger protein A20 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C64 family.

POST-TRANSLATIONAL MODIFICATION

Proteolytically cleaved by MALT1 upon TCR stimulation; disrupts NF-kappa-B inhibitory function and results in increased IL-2 production. It is proposed that only a fraction of TNFAIP3 colocalized with TCR and CBM complex is cleaved, leaving the main TNFAIP3 pool intact.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

A20 is a Cys2/Cys2 zinc finger protein that is induced by a variety of inflammatory stimuli and regulates gene expression. Specifically, A20 is induced by tumor necrosis factor (TNF) and interleukin 1 (IL-1), and acts as a negative regulator of nuclear factor κ B (NFκB) gene expression. By inhibiting NFκB activation, A20 plays a critical role in terminating NFκB responses to various stimuli. Although the C-terminal region of A20 contains seven zinc finger domains, only four of these domains are required for in vitro inhibition of TNF-induced NFκB activation. A20 also interacts with several other proteins, such as TRAF2, TRAF6 and IkB kinase (IKK) γ protein, and can thereby inhibit cell death. TXBP151, a novel A20-binding protein, may mediate the anti-apoptotic activity of A20. Involved in the negative feedback regulation of signal transduction, A20 and A20-binding proteins may be useful as novel therapeutic tools in the treatment of a variety of diseases.