TNF alpha Recombinant Rabbit Monoclonal Antibody [PS01-07] (HA722022)
Catalog# HA722022
Rabbit Monoclonal to TNF alpha
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WB
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Human
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Mouse
Western blot analysis of TNF alpha on different lysates with Rabbit anti-TNF alpha antibody (HA722022) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 treated with 100ng/mL LPS for 7 hours, add 300ng/mL BFA for 3 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26/50/17 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
TNF alpha Recombinant Rabbit Monoclonal Antibody [PS01-07] (HA722022)
Immunogen
Recombinant protein within full length protein.
Host
Rabbit
Positive Control
THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate, RAW264.7 treated with 100ng/mL LPS for 7 hours add 300ng/mL BFA for 3 hours cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PS01-07
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 26 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TNF alpha
SYNONYMS
APC1 antibody;APC1 protein antibody;Cachectin antibody;DIF antibody;Differentiation inducing factor antibody;Macrophage cytotoxic factor antibody;Tnf antibody;TNF superfamily member 2 antibody;TNF superfamily, member 2 antibody;TNF, macrophage derived antibody;TNF, monocyte derived antibody;TNF-a antibody;TNF-alpha antibody;TNFA antibody;TNFA_HUMAN antibody;TNFSF2 antibody;Tumor necrosis factor (TNF superfamily member 2) antibody;Tumor necrosis factor alpha antibody;Tumor necrosis factor antibody;Tumor necrosis factor ligand superfamily member 2 antibody;Tumor Necrosis Factor, Membrane Form antibody;Tumor necrosis factor, soluble form antibody
SUBCELLULAR LOCATION
Cell membrane, Secreted.
FUNCTION
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.
CITATIONS
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Sauchinone alleviates dextran sulfate sodium-induced ulcerative colitis via NAD(P)H dehydrogenase [quinone] 1/NF-kB pathway and gut microbiota
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WB
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Mouse
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Citation
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Dual-engineered,“Trojanized” macrophages bio-modally eradicate tumors through biologically and photothermally deconstructing cancer cells in an on-demand, NIR-commanded, self-explosive manner
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WB
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Mouse
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Citation
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Osteoclast-Derived Exosomal miR-5134-5p Interferes with Alveolar Bone Homeostasis by Targeting the JAK2/STAT3 Axis
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IHC
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Mouse
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Citation
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