PRODUCT CODE: ER1803-48

Tmem248 Rabbit Polyclonal Antibody (ER1803-48)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Tmem248 on SkBr3 (1) and K562 cells lysates using anti-Tmem248 antibody.
  • Western blot analysis of Tmem248 on SkBr3 (1) and K562 cells lysates using anti-Tmem248 antibody.
  • ICC staining Tmem248 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tmem248 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tmem248 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of SH-SY-5Y cells with Tmem248 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Tmem248 on SkBr3 (1) and K562 cells lysates using anti-Tmem248 antibody.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Tmem248 Rabbit Polyclonal Antibody (ER1803-48)

Immunogen

Synthetic peptide within human tmem248 aa 265-314 / 314.

Host

Rabbit

Positive Control

SkBr3, K562, SH-SY-5Y, HUVEC, SiHa.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

35/22 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tmem248

SEQUENCE SIMILARITIES

Belongs to the TMEM248 family.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

This gene is thought to be involved in apoptosis, and may also be involved in hematopoietic development and differentiation. The use of alternative splice sites and promotors result in multiple transcript variants encoding different isoforms. Promotes osteoblast cell differentiation and terminal mineralization. Plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway.