TMEM119 Rabbit Polyclonal Antibody (HA500260)
Catalog# HA500260
Rabbit Polyclonal to TMEM119
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WB
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IF-Cell
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FC
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Human
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Mouse
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse hippocampus tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 45 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500260) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
TMEM119 Rabbit Polyclonal Antibody (HA500260)
Immunogen
Synthetic peptide within human tmem119 aa 11-52 / 283.
Host
Rabbit
Positive Control
Mouse brain tissue lysate, mouse hippocampus tissue lysate, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:50-1:100
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FC
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1:100-1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 29 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TMEM119
SYNONYMS
OBIF antibody;Osteoblast induction factor antibody;PSEC0199 antibody;Transmembrane protein 119 antibody;UNQ731/PRO1415 antibody
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane, Cell membrane, Cytoplasm.
FUNCTION
Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts. May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation.
CITATIONS
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Jingjing Li,et al
Exploring the mechanism of Taohong Siwu Decoction in treating ischemic stroke injury via the circDnajc1/miR-27a-5p/C1qc signaling axis
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WB;IF
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Rat
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Citation
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