PRODUCT CODE: ER2001-37

TMEM119 Rabbit Polyclonal Antibody (ER2001-37)

Applications

  • WB

REACTIVITY

  • Human

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Western blot analysis of TMEM119 on SHSY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of TMEM119 on SHSY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TMEM119 on SHSY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TMEM119 Rabbit Polyclonal Antibody (ER2001-37)

Immunogen

Synthetic peptide within human transmembrane protein 119 aa 120-170.

Host

Rabbit

Positive Control

SHSY5Y cell lysates.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

Predicted band size: 29 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

TMEM119

SYNONYMS

OBIF antibody; Osteoblast induction factor antibody; PSEC0199 antibody; Transmembrane protein 119 antibody; UNQ731/PRO1415 antibody

SUBCELLULAR LOCATION

Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane.

FUNCTION

Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts . May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation (By similarity).