TLR7 Rabbit Polyclonal Antibody (ER30606)
Catalog# ER30606
Rabbit Polyclonal to TLR7
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 121 kDa
Observed band size: 140 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30606) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
TLR7 Rabbit Polyclonal Antibody (ER30606)
Immunogen
Synthetic peptide within mouse tlr7 aa 597-640
Host
Rabbit
Positive Control
Raji cell lysate, Jurkat cell lysate, human tonsil tissue, human lung tissue, mouse lung tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 121 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TLR7
SYNONYMS
PRO285 antibody;TLR 7 antibody;Tlr7 antibody;TLR7_HUMAN antibody;Toll like receptor 7 antibody;Toll-like receptor 7 antibody;UNQ248 antibody
SEQUENCE SIMILARITIES
Belongs to the Toll-like receptor family.
TISSUE SPECIFICITY
Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane.
FUNCTION
Toll-like receptor 7, also known as TLR7, is a protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. It is a member of the toll-like receptor (TLR) family and detects single stranded RNA. The TLR family plays an important role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on the human X chromosome. TLR7 recognizes single-stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages and dendritic cells. TLR7 recognizes single-stranded RNA of viruses such as HIV and HCV. TLR7 can recognize GU-rich single-stranded RNA. However, the presence of GU-rich sequences in the single-stranded RNA is not sufficient to stimulate TLR7.
CITATIONS
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Xu Cheng,et al
Nanomaterial‐Mediated Reprogramming of Macrophages to Inhibit Refractory Muscle Fibrosis
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IF
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Mouse
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Citation
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Shao, Y., Chen, T., Zheng, X., Yang, S., Xu, K., Chen, X., Xu, F., Wang, L., Shen, Y., Wang, T., Zhang, M., Hu, W., Ye, C., Yu, X., Shao, J., & Zheng, S.
Colorectal cancer-derived small extracellular vesicles establish an inflammatory premetastatic niche in liver metastasis
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IF
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Mouse
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Citation
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