Positive control:
Lane 1: mouse brain tissue lysate
Lane 2: U937 cell lysate
Lane 3: SH-SY5Y cell lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Tissue Factor Recombinant Rabbit Monoclonal Antibody [SN20-16] (ET1611-95)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Mouse brain tissue lysate, U937 cell lysate, SH-SY5Y cell lysate, PANC-1, SHG-44, SH-SY5Y, human pancreas tissue, human breast carcinoma tissue, mouse pancreas tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN20-16
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
50 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC/IF
-
1:100-1:500
-
IHC-P
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
Tissue Factor
SYNONYMS
CD142 antibody; CD142 antigen antibody; Coagulation factor III (thromboplastin tissue factor) antibody; Coagulation factor III antibody; F3 antibody; FLJ17960 antibody; TF antibody; TF_HUMAN antibody; TFA antibody; Thromboplastin antibody; Tissue factor antibody
SEQUENCE SIMILARITIES
Belongs to the tissue factor family.
TISSUE SPECIFICITY
Lung, placenta and pancreas.
SUBCELLULAR LOCATION
Membrane,screted.
FUNCTION
Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Coagulation Factor V (Factor V, FV, proaccelerin, labile factor) is a 2196 amino acid, single chain glycoprotein that is cleaved by Thrombin to yield an active, Ca2+-dependent dimer that is essential to the blood coagulation cascade. Together with catalytic Factor Xa and Ca2+ on the surface of platelets or endothelial cells, Factor Va coordinates into a Prothrombinase complex, which mediates proteolysis of Prothrombin into active Thrombin. Tissue factor (TF), also designated coagulation Factor III is a cell surface glycoprotein that enables cells to initiate blood coagulation cascades. It functions as a high-affinity receptor for coagulation Factor VII.