TIMP4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH20-91] (HA751773)
Catalog# HA751773
Rabbit Monoclonal to TIMP4
Tested for
-
WB
-
FC
Reacts with
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Human
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Mouse
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Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TIMP4 on different lysates with Rabbit anti-TIMP4 antibody (HA751773) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 5: HEK-293 cell lysate (20 µg/Lane)
Lane 6: BxPC-3 cell lysate (20 µg/Lane)
Lane 7: Raji cell lysate (20 µg/Lane)
Lane 8: COS-1 cell lysate (20 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751773) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 5: HEK-293 cell lysate (20 µg/Lane)
Lane 6: BxPC-3 cell lysate (20 µg/Lane)
Lane 7: Raji cell lysate (20 µg/Lane)
Lane 8: COS-1 cell lysate (20 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751773) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.