PRODUCT CODE: ET1703-81

TIMP2 Recombinant Rabbit Monoclonal Antibody [JM87-10] (ET1703-81)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of TIMP2 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of TIMP2 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of TIMP2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-81, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of TIMP2 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

TIMP2 Recombinant Rabbit Monoclonal Antibody [JM87-10] (ET1703-81)

Immunogen

Synthetic peptide within c-terminal human timp2.

Host

Rabbit

Positive Control

K562, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM87-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

TIMP2

SYNONYMS

CSC 21K antibody; CSC-21K antibody; CSC21K antibody; Metalloproteinase inhibitor 2 antibody; Metalloproteinase inhibitor 2 precursor antibody; TIMP 2 antibody; TIMP metallopeptidase inhibitor 2 antibody; TIMP-2 antibody; TIMP2 antibody; TIMP2_HUMAN antibody; Tissue Inhibitor of Metalloproteinase 2 antibody; Tissue inhibitor of metalloproteinases 2 antibody

SEQUENCE SIMILARITIES

Belongs to the protease inhibitor I35 (TIMP) family.

POST-TRANSLATIONAL MODIFICATION

The activity of TIMP2 is dependent on the presence of disulfide bonds.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases 1, 2, 3 and 4) complex with metalloproteinases such as collagenases, gelatin-ases and stromelysins, resulting in irreversible inactivation of the metallopro-teinase. TIMP-1 has been found to be identical to EPA (erythroid-potentiation activity). Parathyroid hormone has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus and remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart, with low levels expressed in liver, brain, lung, thymus and spleen.