PRODUCT CODE: ET7110-24

TIMM50 (Tim50) Recombinant Rabbit Monoclonal Antibody [JE51-61] (ET7110-24)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

-
+
Western blot analysis of TIMM50 (Tim50) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A549 cell lysate<br />
Lane 2: K562 cell lysate<br />
Lane 3: MCF-7 cell lysate
  • Western blot analysis of TIMM50 (Tim50) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A549 cell lysate<br />
Lane 2: K562 cell lysate<br />
Lane 3: MCF-7 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-TIMM50 (Tim50) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24, 1/50)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-TIMM50 (Tim50) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24, 1/50)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-TIMM50 (Tim50) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-24, 1/50)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of TIMM50 (Tim50) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: K562 cell lysate
Lane 3: MCF-7 cell lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

TIMM50 (Tim50) Recombinant Rabbit Monoclonal Antibody [JE51-61] (ET7110-24)

Immunogen

Synthetic peptide within n-terminal human tim50.

Host

Rabbit

Positive Control

A549, K562, MCF-7, human liver carcinoma tissue, human colon carcinoma tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE51-61

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

TIMM50 (Tim50)

SYNONYMS

Homolog of yeast Tim50 antibody; Import inner membrane translocase subunit TIM 50 antibody; Import inner membrane translocase subunit TIM50 antibody; Import inner membrane translocase subunit TIM50 mitochondrial antibody; MGC102733 antibody; Mitochondrial import inner membrane translocase subunit TIM50 antibody; TIM 50 antibody; TIM 50L antibody; TIM50 antibody; Tim50 like protein antibody; TIM50_HUMAN antibody; TIM50L antibody; TIMM 50 antibody; TIMM50 antibody; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody; Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody; Translocase of inner mitochondrial membrane 50 homolog antibody; Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody

SEQUENCE SIMILARITIES

Belongs to the TIM50 family.

TISSUE SPECIFICITY

Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).

SUBCELLULAR LOCATION

Mitochondrion inner membrane, nucleus speckle.

FUNCTION

Mitochondrial import inner membrane translocase subunit TIM50 is an enzyme that in humans is encoded by the TIMM50 gene. Tim50 is a subunit of the Tim23 translocase complex in the inner mitochondrial membrane. Mutations in TIMM50 can lead to epilepsy, severe intellectual disability, and 3-methylglutaconic aciduria. TIMM50 expression is increased in breast cancer cells and decreased in hypertrophic hearts. The TIMM50 gene is located on the q arm of chromosome 19 in position 13.2 and spans 13,373 base pairs. The gene produces a 39.6 kDa protein composed of 353 amino acids. This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex, which mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. The Tim50 protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This gene in human cells results in the release of cytochrome c and apoptosis. This protein plays a role in maintaining the membrane permeability barrier. The intermembrane space domain of Tim50 induces the translocation pore of the TIM23 channel to close.