Applications
-
WB
-
ICC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
TIE-1 Rabbit Polyclonal Antibody (0804-11)
Immunogen
Synthetic peptide within human tie-1 aa 23-72 / 1,138.
Host
Rabbit
Positive Control
K562, HepG2, SW620.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
125kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
TIE-1
SYNONYMS
JKT 14 antibody; JTK14 antibody; TIE antibody; Tie1 antibody; TIE1_HUMAN antibody; Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody; Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody; Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody; Tyrosine protein kinase receptor Tie 1 antibody; Tyrosine-protein kinase receptor Tie-1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
TISSUE SPECIFICITY
Specifically expressed in developing vascular endothelial cells.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
SUBCELLULAR LOCATION
Cell membrane. Membrane.
FUNCTION
The Tie-1 receptor is an endothelial specific cell surface tyrosine kinase that is indispensable for endothelial functions. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Tie-1 and Tie-2 are receptor tyrosine kinases (RTKs) that are exclusively expressed in endothelial cells and play important roles in endothelial cell biology.