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PRODUCT CODE: 0804-11

TIE-1 Rabbit Polyclonal Antibody (0804-11)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Tie-1 on K562 cell lysate using anti-Tie-1 antibody at 1/2,000 dilution.
  • Western blot analysis of Tie-1 on K562 cell lysate using anti-Tie-1 antibody at 1/2,000 dilution.
  • ICC staining Tie-1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tie-1 in SW620 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Tie-1 on K562 cell lysate using anti-Tie-1 antibody at 1/2,000 dilution.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TIE-1 Rabbit Polyclonal Antibody (0804-11)

Immunogen

Synthetic peptide within n-terminal human tie-1.

Host

Rabbit

Positive Control

K562, HepG2, SW620.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

125kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

P35590

PROTEIN NAME

TIE-1

SYNONYMS

JKT 14 antibody; JTK14 antibody; TIE antibody; Tie1 antibody; TIE1_HUMAN antibody; Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody; Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody; Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody; Tyrosine protein kinase receptor Tie 1 antibody; Tyrosine-protein kinase receptor Tie-1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

TISSUE SPECIFICITY

Specifically expressed in developing vascular endothelial cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.

SUBCELLULAR LOCATION

Cell membrane. Membrane.

FUNCTION

The Tie-1 receptor is an endothelial specific cell surface tyrosine kinase that is indispensable for endothelial functions. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Tie-1 and Tie-2 are receptor tyrosine kinases (RTKs) that are exclusively expressed in endothelial cells and play important roles in endothelial cell biology.