PRODUCT CODE: ET1702-31

Thymidine Kinase 1 Recombinant Rabbit Monoclonal Antibody [JF0970] (ET1702-31)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of Thymidine Kinase 1 on different lysates using anti-Thymidine Kinase 1 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: 293T             <br />
Lane 2: Hela
  • Western blot analysis of Thymidine Kinase 1 on different lysates using anti-Thymidine Kinase 1 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: 293T             <br />
Lane 2: Hela
  • ICC staining Thymidine Kinase 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Thymidine Kinase 1 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Thymidine Kinase 1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Thymidine Kinase 1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Thymidine Kinase 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Thymidine Kinase 1 on different lysates using anti-Thymidine Kinase 1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Hela

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Thymidine Kinase 1 Recombinant Rabbit Monoclonal Antibody [JF0970] (ET1702-31)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, SW480, Hela, 293T, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0970

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Thymidine Kinase 1

SYNONYMS

cytosolic antibody; KITH_HUMAN antibody; Thymidine kinase 1 antibody; Thymidine kinase 1 soluble antibody; Thymidine kinase 1 soluble isoform antibody; Thymidine kinase antibody; Thymidine kinase cytosolic antibody; TK 1 antibody; TK 2 antibody; TK1 antibody; Tk1a antibody; Tk1b antibody; TK2 antibody

SEQUENCE SIMILARITIES

Belongs to the thymidine kinase family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Ser-13 in mitosis.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division. Because of this, Thymidine Kinase can be used as a proliferation marker in the diagnosis, treatment, and follow-up of malignant diseases, especially hematological malignancies. Thymidine Kinase may be observed as a monomer, dimer, trimer or tetramer.