PRODUCT CODE: ET7107-92

Thrombomodulin Recombinant Rabbit Monoclonal Antibody [JB48-23] (ET7107-92)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of Thrombomodulin on A431 cell using anti-Thrombomodulin antibody at 1/500 dilution.
  • Western blot analysis of Thrombomodulin on A431 cell using anti-Thrombomodulin antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.
Western blot analysis of Thrombomodulin on A431 cell using anti-Thrombomodulin antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Thrombomodulin Recombinant Rabbit Monoclonal Antibody [JB48-23] (ET7107-92)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human lung cancer tissue, human placenta tissue, A431.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB48-23

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Thrombomodulin

SYNONYMS

AHUS 6 antibody; AHUS6 antibody; BDCA 3 antibody; BDCA3 antibody; CD 141 antibody; CD141 antibody; CD141 antigen antibody; Fetomodulin antibody; Thbd antibody; THPH12 antibody; THRM antibody; Thrombomodulin antibody; TM antibody; TRBM_HUMAN antibody

TISSUE SPECIFICITY

Endothelial cells are unique in synthesizing thrombomodulin.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.; The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Thrombomodulin (TM), also called CD141, is a type I membrane receptor that is specific to endothelial cells. TM has a cysteine-rich extracellular domain with six EGF-like regions. It forms a complex with Thrombin, which activates Protein C to generate activated Protein C (APC), an anticoagulant enzyme. APC together with Protein S inhibits coagulation by inactivating Factors Va and VIIIa. Deletion of the TM gene results in embryonic lethality in mice.