PRODUCT CODE: ET1702-90

Thioredoxin Recombinant Rabbit Monoclonal Antibody [JF101-8] (ET1702-90)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: 293T
  • Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: 293T
  • Flow cytometric analysis of Hela cells with TRX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Thioredoxin Recombinant Rabbit Monoclonal Antibody [JF101-8] (ET1702-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF101-8

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

12 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Thioredoxin

SYNONYMS

ADF antibody; ATL derived factor antibody; ATL-derived factor antibody; DKFZp686B1993 antibody; MGC61975 antibody; SASP antibody; Surface associated sulphydryl protein antibody; Surface-associated sulphydryl protein antibody; testicular tissue protein Li 199 antibody; THIO_HUMAN antibody; Thioredoxin antibody; thioredoxin delta 3 antibody; TRDX antibody; TRX 1 antibody; Trx antibody; TRX1 antibody; TXN antibody; TXN delta 3 antibody; TXN protein antibody; zgc:92903 antibody

SEQUENCE SIMILARITIES

Belongs to the thioredoxin family.

POST-TRANSLATIONAL MODIFICATION

In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.; In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm, Secreted.

FUNCTION

Thioredoxin (Trx) is a redox protein that is found in several species, such as bacteria, plants and mammals, and contains a conserved active site, consisting of Trp-Cys-Gly-Pro-Cys. Trx has several biological functions. It acts as a hydrogen donor for ribonucleotide reductase, which is critical for DNA synthesis, and modulates the DNA-binding activity of several transcription factors, including NFkB, AP-1, p53, TFIIIC and glucocorticoid receptor. Trx also stimulates cell growth, is an inhibitor of apoptosis and plays a role in the protection against oxidative stress. Drugs that inhibit Trx have antitumor activity, suggesting that Trx is involved in a variety of human diseases, including cancer. TrxR is a ubiquitously expressed flavoprotein that catalyzes the NADPH-dependent reduction of Trx as well as several other oxidized cellular components.