PRODUCT CODE: ET1702-56

TGN46 Recombinant Rabbit Monoclonal Antibody [JF1-024] (ET1702-56)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of TGN46 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of TGN46 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGN46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of TGN46 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

TGN46 Recombinant Rabbit Monoclonal Antibody [JF1-024] (ET1702-56)

Immunogen

Synthetic peptide within human tgn46 aa 43-79 / 437l5l562:l576.

Host

Rabbit

Positive Control

NIH/3T3, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF1-024

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

95 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IP

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

TGN46

SYNONYMS

TGN 46 antibody; TGN 51 antibody; TGN38 antibody; TGN38 homolog antibody; TGN46 antibody; TGN48 antibody; TGN51 antibody; TGOLN2 antibody; TGON2_HUMAN antibody; Trans Golgi network integral membrane protein 2 antibody; Trans golgi network protein 2 antibody; Trans Golgi network protein TGN51 antibody; Trans-Golgi network integral membrane protein 2 antibody; Trans-Golgi network protein TGN51 antibody; TTGN2 antibody

TISSUE SPECIFICITY

Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.

SUBCELLULAR LOCATION

Cell membrane, Golgi apparatus.

FUNCTION

TGN38 (trans-Golgi network protein 2) is a type I integral membrane protein that constitutively cycles between the TGN and plasma membrane where it partitions nascent proteins into carrier vesicles for transport to appropriate destinations in the cell. The cytosolic domain of TGN38 interacts with AP2 Clathrin adaptor complexes via the tyrosine-containing motif (SDYQRL) to direct internalization from the plasma membrane. N- and O-linked oligosaccharide chains attach to the core TGN38 protein to produce a protein present in brain, lung and kidney.