PRODUCT CODE: ET1702-56

TGN46 Recombinant Rabbit Monoclonal Antibody [JF1-024] (ET1702-56)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

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+
Western blot analysis of TGN46 on NIH/3T3 cells lysates using anti-TGN46 antibody at 1/1,000 dilution.
  • Western blot analysis of TGN46 on NIH/3T3 cells lysates using anti-TGN46 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGN46 antibody. Counter stained with hematoxylin.
Western blot analysis of TGN46 on NIH/3T3 cells lysates using anti-TGN46 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

TGN46 Recombinant Rabbit Monoclonal Antibody [JF1-024] (ET1702-56)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF1-024

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

95 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IP

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

TGN46

SYNONYMS

TGN 46 antibody; TGN 51 antibody; TGN38 antibody; TGN38 homolog antibody; TGN46 antibody; TGN48 antibody; TGN51 antibody; TGOLN2 antibody; TGON2_HUMAN antibody; Trans Golgi network integral membrane protein 2 antibody; Trans golgi network protein 2 antibody; Trans Golgi network protein TGN51 antibody; Trans-Golgi network integral membrane protein 2 antibody; Trans-Golgi network protein TGN51 antibody; TTGN2 antibody

TISSUE SPECIFICITY

Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.

SUBCELLULAR LOCATION

Cell membrane, Golgi apparatus.

FUNCTION

TGN38 (trans-Golgi network protein 2) is a type I integral membrane protein that constitutively cycles between the TGN and plasma membrane where it partitions nascent proteins into carrier vesicles for transport to appropriate destinations in the cell. The cytosolic domain of TGN38 interacts with AP2 Clathrin adaptor complexes via the tyrosine-containing motif (SDYQRL) to direct internalization from the plasma membrane. N- and O-linked oligosaccharide chains attach to the core TGN38 protein to produce a protein present in brain, lung and kidney.