PRODUCT CODE: ER1706-28

TGM6 Rabbit Polyclonal Antibody (ER1706-28)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TGM6 on different tissue lysates using anti-TGM6 antibody at 1/100 dilution.<br />
  Positive control:<br />
  Lane 1 : Mouse brain <br />
  Lane 2 : Mouse brain<br />
  Lane 3 : Mouse spleen <br />
  Lane 4 : Mouse spleen<br />
  Lane 5 : Mouse testis
  • Western blot analysis of TGM6 on different tissue lysates using anti-TGM6 antibody at 1/100 dilution.<br />
  Positive control:<br />
  Lane 1 : Mouse brain <br />
  Lane 2 : Mouse brain<br />
  Lane 3 : Mouse spleen <br />
  Lane 4 : Mouse spleen<br />
  Lane 5 : Mouse testis
  • ICC staining TGM6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TGM6 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TGM6 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGM6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TGM6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with TGM6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of TGM6 on different tissue lysates using anti-TGM6 antibody at 1/100 dilution.
Positive control:
Lane 1 : Mouse brain
Lane 2 : Mouse brain
Lane 3 : Mouse spleen
Lane 4 : Mouse spleen
Lane 5 : Mouse testis

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TGM6 Rabbit Polyclonal Antibody (ER1706-28)

Immunogen

Peptide

Host

Rabbit

Positive Control

293T, A549, SH-SY5Y, rat brain tissue, human kidney tissue, mouse testis tissue, mouse brain tissue, mouse spleen tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

79/71 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

TGM6

SYNONYMS

Protein-glutamine gamma-glutamyltransferase 6 antibody; TG6 antibody; TGase Y antibody; TGase-3-like antibody; TGase-6 antibody; TGM3L_HUMAN antibody; TGM6 antibody; TGY antibody; Transglutaminase Y antibody; Transglutaminase-3-like antibody; Transglutaminase-6 antibody

SEQUENCE SIMILARITIES

Belongs to the transglutaminase superfamily. Transglutaminase family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Transglutaminase 6 is a protein that in humans is encoded by the TGM6 gene. The protein encoded by this gene belongs to the transglutaminase superfamily. It catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Mutations in this gene are associated with spinocerebellar ataxia type 35 (SCA35). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.