TGF beta Receptor II Recombinant Rabbit Monoclonal Antibody [PSH01-50] (HA721693)
Catalog# HA721693
Rabbit Monoclonal to TGF beta Receptor II
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TGF beta Receptor II on different lysates with Rabbit anti-TGF beta Receptor II antibody (HA721693) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: A549 cell lysate
Lane 3: HT-29 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65-80 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721693) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: A549 cell lysate
Lane 3: HT-29 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65-80 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721693) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.