TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PSH18-59] (HA724012)
Catalog# HA724012
Rabbit Monoclonal to TGF beta 1
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
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Mouse
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Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (HA724012) at 1/5,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: Raji cell lysate
Lane 3: Saos-2 cell lysate
Lane 4: 786-0 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724012) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate
Lane 2: Raji cell lysate
Lane 3: Saos-2 cell lysate
Lane 4: 786-0 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724012) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.