TFIIE alpha Recombinant Rabbit Monoclonal Antibody [JE66-41] (HA721376)
Catalog# HA721376
Rabbit Monoclonal to TFIIE alpha
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TFIIE alpha on different lysates with Rabbit anti-TFIIE alpha antibody (HA721376) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: Daudi cell lysate (15 µg/Lane)
Lane 4: HeLa cell lysate (15 µg/Lane)
Lane 5: A431 cell lysate (15 µg/Lane)
Lane 6: A549 cell lysate (15 µg/Lane)
Lane 7: HepG2 cell lysate (15 µg/Lane)
Lane 8: HEK-293 cell lysate (15 µg/Lane)
Lane 9: NIH/3T3 cell lysate (15 µg/Lane)
Lane 10: MCF7 cell lysate (15 µg/Lane)
Lane 11: Mouse testis tissue lysate (30 µg/Lane)
Lane 12: Rat testis tissue lysate (30 µg/Lane)
Predicted band size: 49.5 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721376) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: Daudi cell lysate (15 µg/Lane)
Lane 4: HeLa cell lysate (15 µg/Lane)
Lane 5: A431 cell lysate (15 µg/Lane)
Lane 6: A549 cell lysate (15 µg/Lane)
Lane 7: HepG2 cell lysate (15 µg/Lane)
Lane 8: HEK-293 cell lysate (15 µg/Lane)
Lane 9: NIH/3T3 cell lysate (15 µg/Lane)
Lane 10: MCF7 cell lysate (15 µg/Lane)
Lane 11: Mouse testis tissue lysate (30 µg/Lane)
Lane 12: Rat testis tissue lysate (30 µg/Lane)
Predicted band size: 49.5 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721376) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.