TFII I Recombinant Rabbit Monoclonal Antibody [JE56-20] (ET7111-10)
Catalog# ET7111-10
Rabbit Monoclonal to TFII I
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TFII I on different lysates with Rabbit anti-TFII I antibody (ET7111-10) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 112 kDa
Observed band size: 112/150 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-10) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 112 kDa
Observed band size: 112/150 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-10) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.