PRODUCT CODE: R1309-16

TESPA1 Rabbit Polyclonal Antibody (R1309-16)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TESPA1 on different lysates using anti-TESPA1 antibody at 1/1,000 dilution.
  • Western blot analysis of TESPA1 on different lysates using anti-TESPA1 antibody at 1/1,000 dilution.
  • ICC staining TESPA1 in A172 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with TESPA1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of TESPA1 on different lysates using anti-TESPA1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TESPA1 Rabbit Polyclonal Antibody (R1309-16)

Immunogen

Synthetic peptide within human tespa1 aa 226-270.

Host

Rabbit

Positive Control

Human thymus, mouse thymus, human liver, A431, A172, rat brain, human lung cancer, mouse brain, mouse pancreas, HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

50kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

TESPA1

SYNONYMS

HSPC257 antibody; K0748_HUMAN antibody; KIAA0748 antibody; Protein TESPA1 antibody; TESPA1 – thymocyte expressed, positive selection associated 1 antibody; TESPA1 antibody; thymocyte expressed, positive selection associated 1 antibody; Uncharacterized protein KIAA0748 antibody

POST-TRANSLATIONAL MODIFICATION

May be phosphorylated in response to store-operated Ca(+2) entry.

SUBCELLULAR LOCATION

Cytoplasm. Membrane.

FUNCTION

TESPA1 (Thymocyte-expressed positive selection-associated protein 1) is required for the development and maturation of T-cells, its function being essential for the late stages of thymocyte development. It plays a role in T-cell antigen receptor (TCR)-mediated activation of the ERK and NFAT signaling pathways, possibly by serving as a scaffolding protein that promotes the assembly of the LAT signalosome in thymocytes.