PRODUCT CODE: ET1608-50

Tenascin C Recombinant Rabbit Monoclonal Antibody [SU36-01] (ET1608-50)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tenascin C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tenascin C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Tenascin C was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-50, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tenascin C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Tenascin C Recombinant Rabbit Monoclonal Antibody [SU36-01] (ET1608-50)

Immunogen

Synthetic peptide within human tenascin c aa 2,152-2,201 / 2,201.

Host

Rabbit

Positive Control

Human tonsil tissue, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU36-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

241 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Tenascin C

SYNONYMS

150 225 antibody; Cytotactin antibody; Glioma associated extracellular matrix antigen antibody; Glioma-associated-extracellular matrix antigen antibody; GMEM antibody; GP 150 225 antibody; GP 150-225 antibody; GP antibody; Hexabrachion antibody; HXB antibody; JI antibody; MGC167029 antibody; Miotendinous antigen antibody; Myotendinous antigen antibody; Neuronectin antibody; TENA_HUMAN antibody; Tenascin antibody; Tenascin-C antibody; Tenascin-C isoform 14/AD1/16 antibody; TenascinC antibody; TN antibody; TN C antibody; TN-C antibody; TNC antibody

SEQUENCE SIMILARITIES

Belongs to the tenascin family.

SUBCELLULAR LOCATION

Extracellular matrix.

FUNCTION

The tenascin family of extracellular matrix proteins includes Tenascin-C (also designated cytotactin or Tenascin), Tenascin-R (also designated restrictin, TN-R or janusin) and Tenascin-X. Tenascin proteins function as substrate-adhesion molecules (SAMs) and are involved in regulating numerous developmental processes, such as morphogenetic cell migration and organogenesis. The tenascin family proteins arise from various splicing events in the region of coding for FNIII repeats. Tenascin-C and Tenascin-X are expressed in several tissues during embryogenesis and in adult tissues undergoing active remodeling, such as healing wounds and tumors. Tenascin-R is expressed on the surface of neurons and glial cells.