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PRODUCT CODE: ER1706-98

TEAD4 Rabbit Polyclonal Antibody (ER1706-98)

Applications

  • WB

  • FC

REACTIVITY

  • Human

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Western blot analysis of TEAD4 on different lysates using anti-TEAD4 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Hela <br /> Lane 2: Human lung tissue<br /> Lane 3: Human placenta tissue <br /> Lane 4: HepG2
  • Western blot analysis of TEAD4 on different lysates using anti-TEAD4 antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Hela <br /> Lane 2: Human lung tissue<br /> Lane 3: Human placenta tissue <br /> Lane 4: HepG2
  • Flow cytometric analysis of LOVO cells with TEAD4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of TEAD4 on different lysates using anti-TEAD4 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Human lung tissue
Lane 3: Human placenta tissue
Lane 4: HepG2

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TEAD4 Rabbit Polyclonal Antibody (ER1706-98)

Immunogen

Synthetic peptide within human tead4 aa 57-121.

Host

Rabbit

Positive Control

Hela, human lung tissue lysate, human placenta tissue lysate, HepG2, LOVO.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q15561

PROTEIN NAME

TEAD4

SYNONYMS

EFTR 2 antibody; EFTR2 antibody; hRTEF 1B antibody; hRTEF1B antibody; MGC9014 antibody; OTTHUMP00000238119 antibody; OTTHUMP00000238122 antibody; OTTHUMP00000238124 antibody; Related to TEF 1 antibody; Related to TEF1 antibody; Related transcription enhancer factor 1B antibody; RTEF1 antibody; TCF13L1 antibody; TEA domain family member 4 antibody; TEAD 4 antibody; TEAD-4 antibody; TEAD4 antibody; TEAD4_HUMAN antibody; TEF 3 antibody; TEF3 antibody; TEFR 1 antibody; TEFR1 antibody; Transcription factor 13 (SV40 transcriptional enhancer factor) like 1 antibody; Transcription factor 13 like 1 antibody; Transcription factor 13-like 1 antibody; Transcription factor RTEF 1 antibody; Transcription factor RTEF-1 antibody; Transcription factor RTEF1 antibody; Transcriptional enhancer factor 1 related antibody; Transcriptional enhancer factor 3 antibody; Transcriptional enhancer factor TEF 3 antibody; Transcriptional enhancer factor TEF-3 antibody; Transcriptional enhancer factor TEF3 antibody

TISSUE SPECIFICITY

Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

TEF-3, also known as TEAD4 (TEA domain family member 4), RTEF1, EFTR-2, TEFR-1, TCF13L1 or hRTEF-1B, is a 427 amino acid member of the transcriptional enhancer factor (TEF) family of proteins that are characterized by the presence of a TEA DNA-binding domain. Localized to the nucleus and expressed primarily in skeletal muscle, TEF-3 functions as a transcriptional regulator by binding specifically and non-cooperatively to the M-CAT motif found in the promotors of muscle-specific genes, thereby directing their subsequent expression. TEF-3 contains one TEA DNA-binding domain and is expressed as multiple isoforms due to alternative splicing events.