Applications
-
WB
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
TdT Rabbit Polyclonal Antibody (0807-4)
Immunogen
Synthetic peptide within human tdt aa 460-509 / 509.
Host
Rabbit
Positive Control
Jurkat
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Immunogen affinity purified
MOLECULAR WEIGHT
58kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:200
TARGET
UNIPROT #
PROTEIN NAME
TdT
SYNONYMS
Deoxynucleotidyltransferase terminal antibody; DNA nucleotidylexotransferase antibody; DNTT antibody; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody; TDT antibody; TDT_HUMAN antibody; Terminal addition enzyme antibody; Terminal deoxynucleotidyltransferase antibody; Terminal deoxyribonucleotidyltransferase antibody; Terminal transferase antibody
SEQUENCE SIMILARITIES
Belongs to the DNA polymerase type-X family.
SUBCELLULAR LOCATION
Cell nucleus
FUNCTION
Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase expressed in immature lymphocytes of the thymus and bone marrow, as well as certain leukemic cells. The nuclear enzyme TdT plays a critical role in generating immune receptor diversity. During rearrangement of Ig and TCR V region genes, TdT catalyzes the addition of N nucleotides without a DNA template to V-D and D-J junctions in both Ig and TCR as well as the V-J junctions in the TCR (1–3). During the fetal and newborn period, TdT activity is absent, resulting in an N region-deficient Ag receptor repertoire significantly lacking in the diversity observed in the junctional regions of Igs and TCRs of adult animals