TDP43 Recombinant Rabbit Monoclonal Antibody [JM51-10] (ET1703-74)
Catalog# ET1703-74
Rabbit Monoclonal to TDP43
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WB
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IP
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IHC-Fr
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Human
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Mouse
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Rat
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Zebrafish
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: Neuro-2a cell lysate
Lane 4: Mouse spleen tissue lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat spleen tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-74) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
TDP43 Recombinant Rabbit Monoclonal Antibody [JM51-10] (ET1703-74)
Immunogen
Synthetic peptide within human tdp43 aa 354-390 / 414; (2)aa 253-290 / 414.
Host
Rabbit
Positive Control
HeLa cell lysate, K-562 cell lysate, Neuro-2a cell lysate, mouse spleen tissue lysate, mouse brain tissue lysate, rat spleen tissue lysate, rat brain tissue lysate, HeLa, Neuro-2a, human spleen tissue, mouse placenta tissue, human pancreas tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, mouse cerebrum tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM51-10
RRID
APPLICATION DILUTION
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WB
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1:500-1:5,000
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IHC-Fr
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1:200-1:500
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IF-Cell
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1:250-1:500
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IF-Tissue
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1:100
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IHC-P
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1:500
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TDP43
SYNONYMS
ALS10 antibody;OTTHUMP00000002171 antibody;OTTHUMP00000002172 antibody;OTTHUMP00000002173 antibody;TADBP_HUMAN antibody;TAR DNA binding protein 43 antibody;TAR DNA binding protein antibody;TAR DNA-binding protein 43 antibody;TARDBP antibody;TDP 43 antibody;TDP-43 antibody;TDP43 antibody
TISSUE SPECIFICITY
Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
POST-TRANSLATIONAL MODIFICATION
Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Phosphorylated upon cellular stress.; Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.; Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
SUBCELLULAR LOCATION
Cytoplasm, Mitochondrion, Nucleus.
FUNCTION
DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
CITATIONS
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Duan Qingqing,et al
TBK1, a prioritized drug repurposing target for amyotrophic lateral sclerosis: evidence from druggable genome Mendelian randomization and pharmacological verification in vitro
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WB
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Mouse
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Citation
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