PRODUCT CODE: ET7109-79

TCP1 alpha/CCTA Recombinant Rabbit Monoclonal Antibody [JE48-48] (ET7109-79)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

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Western blot analysis of TCP1 alpha/CCTA on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-79, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of TCP1 alpha/CCTA on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-79, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer  tissue using anti-TCP1 alpha/CCTA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-TCP1 alpha/CCTA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-TCP1 alpha/CCTA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of TCP1 alpha/CCTA on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-79, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

TCP1 alpha/CCTA Recombinant Rabbit Monoclonal Antibody [JE48-48] (ET7109-79)

Immunogen

Synthetic peptide within c-terminal human tcp1 alpha.

Host

Rabbit

Positive Control

Daudi, human liver cancer tissue, human skin tissue, human breast tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE48-48

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1,000

  • IHC-P:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

TCP1 alpha/CCTA

SYNONYMS

AI528772 antibody; c-cpn antibody; CCT alpha antibody; CCT antibody; CCT-alpha antibody; CCT1 antibody; Ccta antibody; CCTalpha antibody; D6S230E antibody; MGC133746 antibody; p63 antibody; T complex 1 antibody; T complex protein 1 alpha subunit antibody; T complex protein 1 antibody; T-complex homolog TCP1 antibody; T-complex protein 1 subunit alpha antibody; T-complex protein 1 subunit alpha B antibody; Tailless complex polypeptide 1 antibody; Tailless complex polypeptide 1A antibody; Tailless complex polypeptide 1B antibody; TCP 1 alpha antibody; Tcp-1 antibody; TCP-1-alpha antibody; TCP1 antibody; TCPA_HUMAN antibody; Tp63 antibody; TRic antibody

SEQUENCE SIMILARITIES

Belongs to the TCP-1 chaperonin family.

SUBCELLULAR LOCATION

Cytoplasm. Cytoskeleton.

FUNCTION

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).