Tat-SF1 Recombinant Rabbit Monoclonal Antibody [JE54-89] (ET7110-87)
Catalog# ET7110-87
Rabbit Monoclonal to Tat-SF1
Tested for
-
WB
-
IF-Cell
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IHC-P
React with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Tat-SF1 on different lysates with Rabbit anti-Tat-SF1 antibody (ET7110-87) at 1/500 dilution.
Lane 1: CRC cell lysate
Lane 2: MCF-7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 120 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-87) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: CRC cell lysate
Lane 2: MCF-7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 120 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-87) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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