Lane 1: Taq DNA Polymerase
Lane 2: Taq DNA Polymerase + Anti-Taq DNA Polymerase antibody
Lane 3: Taq DNA Polymerase + Control antibody
Antibody-mediated hot start PCR
Mouse monoclonal primary
Taq DNA Polymerase Mouse Monoclonal Antibody [3C3] (EM1701-49)
Taq dna polymerase
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze/thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein G purified.
Anti-Taq DNA Polymerase Antibody
Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.