PRODUCT CODE: HA500013

TAG1 Rabbit Polyclonal Antibody (HA500013)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of TAG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500013, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse cerebellum tissue lysate<br />
Lane 2: Human brain tissue lysate<br />
Lane 3: Rat cerebellum tissue lysate
  • Western blot analysis of TAG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500013, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse cerebellum tissue lysate<br />
Lane 2: Human brain tissue lysate<br />
Lane 3: Rat cerebellum tissue lysate
  • ICC staining of TAG1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500013, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of TAG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500013, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Human brain tissue lysate
Lane 3: Rat cerebellum tissue lysate

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

TAG1 Rabbit Polyclonal Antibody (HA500013)

Immunogen

Recombinant protein within human tag1 aa 40-230.

Host

Rabbit

Positive Control

Mouse cerebellum tissue lysate, human brain tissue lysate, rat cerebellum tissue lysate, F9.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size: 113 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

TAG1

SYNONYMS

Axonal glycoprotein TAG 1 antibody; Axonal glycoprotein TAG-1 antibody; Axonal glycoprotein TAG1 antibody; Axonin 1 antibody; Axonin-1 antibody; Axonin1 antibody; AXT antibody; CNTN 2 antibody; Cntn2 antibody; CNTN2_HUMAN antibody; contactin 2 (axonal) antibody; Contactin 2 antibody; Contactin-2 antibody; Contactin2 antibody; DKFZp781D102 antibody; MGC157722 antibody; TAG 1 antibody; TAG 564 antibody; TAG564 antibody; TAX 1 antibody; TAX antibody; TAX-1 antibody; TAX1 antibody; Transient axonal glycoprotein 1 antibody; Transiently expressed axonal glycoprotein antibody

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

This gene encodes a member of the contactin family of proteins, part of the immunoglobulin superfamily of cell adhesion molecules. The encoded glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein plays a role in the proliferation, migration, and axon guidance of neurons of the developing cerebellum. A mutation in this gene may be associated with adult myoclonic epilepsy.