TACC3 Recombinant Rabbit Monoclonal Antibody [SY10-06] (ET1605-18)
Catalog# ET1605-18
Rabbit Monoclonal to TACC3
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TACC3 on different lysates with Rabbit anti-TACC3 antibody (ET1605-18) at 1/500 dilution.
Lane 1: HT29 cell lysates
Lane 2: Hela cell lysates
Lysates/proteins at 10 µg/Lane.
Predicted band size: 90 kDa
Observed band size: 140 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-18) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: HT29 cell lysates
Lane 2: Hela cell lysates
Lysates/proteins at 10 µg/Lane.
Predicted band size: 90 kDa
Observed band size: 140 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-18) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.