PRODUCT CODE: ET1605-18

TACC3 Recombinant Rabbit Monoclonal Antibody [SY10-06] (ET1605-18)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis of TACC3 on different lysates using anti-TACC3 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: HT29                <br />
Lane 2: Hela
  • Western blot analysis of TACC3 on different lysates using anti-TACC3 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: HT29                <br />
Lane 2: Hela
  • ICC staining TACC3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TACC3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TACC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TACC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TACC3 antibody. Counter stained with hematoxylin.
Western blot analysis of TACC3 on different lysates using anti-TACC3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HT29
Lane 2: Hela

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

TACC3 Recombinant Rabbit Monoclonal Antibody [SY10-06] (ET1605-18)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HT29, Hela, HepG2, human tonsil tissue, human spleen tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY10-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

140 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

TACC3

SYNONYMS

ERIC 1 antibody; ERIC-1 antibody; ERIC1 antibody; MGC117382 antibody; MGC133242 antibody; OTTHUMP00000113796 antibody; TACC3 antibody; TACC3_HUMAN antibody; Transforming acidic coiled coil containing protein 3 antibody; Transforming acidic coiled-coil-containing protein 3 antibody

SEQUENCE SIMILARITIES

Belongs to the TACC family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

TACC1 (transforming acidic coiled coil gene 1) is one of three TACC family members, which are thought to be involved in breast tumorigenesis. TACC1 is located on 8p11 chromosomal region that is amplified in approximately 15% of all breast tumor samples. The short arm of chromosome 8 also contains FGFR1 whose expression is enhanced in most breast cancer tumors. TACC family members, TACC1, TACC2, and TACC3, map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. Also, TACC1 contains a conserved C-terminal region as in the Drosophila homolog, D-TACC. It has been shown that D-TACC is necessary for normal spindle function, and the mammalian TACC proteins appears to interact with centrosomes and microtubules in a similar manner.