PRODUCT CODE: ER1802-65

T-bet Rabbit Polyclonal Antibody (ER1802-65)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of T-bet on mouse marrow tissue lysate using anti-T-bet antibody at 1/500 dilution.
  • Western blot analysis of T-bet on mouse marrow tissue lysate using anti-T-bet antibody at 1/500 dilution.
  • Flow cytometric analysis of Jurkat cells with T-bet antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of T-bet on mouse marrow tissue lysate using anti-T-bet antibody at 1/500 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

T-bet Rabbit Polyclonal Antibody (ER1802-65)

Immunogen

Synthetic peptide within human t-bet aa 486-535 / 535.

Host

Rabbit

Positive Control

Mouse marrow tissue lysate, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

T-bet

SYNONYMS

T bet antibody; T box 21 antibody; T box expressed in T cells antibody; T box protein 21 antibody; T box transcription factor TBX21 antibody; T cell specific T box transcription factor antibody; T cell specific T box transcription factor T bet antibody; T PET antibody; T-box protein 21 antibody; T-box transcription factor TBX21 antibody; T-cell-specific T-box transcription factor T-bet antibody; TBET antibody; TBLYM antibody; TBX 21 antibody; Tbx21 antibody; TBX21_HUMAN antibody TPET antibody; Transcription factor TBLYM antibody

TISSUE SPECIFICITY

T-cell specific. Expressed in regulatory T (TReg) cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylations at Ser-52, Tyr-76, Ser-224 and Ser-508 are regulated by mTORC1. Phosphorylation at Tyr-525 is essential for its interaction GATA3. Phosphorylation at Tyr-219, Tyr-265 and Tyr-304 enhances its transcriptional activator activity. Phosphorylation at Thr-302 is required for its interaction with NFATC2.; Ubiquitinated at Lys-313, leading to its degradation by the proteasome. Ubiquitination is essential for controlling protein stability, binding to the T-box-binding element of the IFN-gamma promoter, and for interaction with NFATC2 through induction of phosphorylation at Thr-302. Deubiquitinated by USP10 leading to its stabilization (By similarity).

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T box expressed in T cells) is a Th1-specific T box transcription factor that controls the expres-sion of the Th1 cytokine, IFN-γ. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. T-bet is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.