SYVN1 Recombinant Rabbit Monoclonal Antibody [JE44-67] (HA721874)
Catalog# HA721874
Rabbit Monoclonal to SYVN1
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WB
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IF-Cell
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FC
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Human
Lane 1: HEK-293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Raji cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721874) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SYVN1 Recombinant Rabbit Monoclonal Antibody [JE44-67] (HA721874)
Immunogen
Synthetic peptide within human syvn1 aa 150-250.
Host
Rabbit
Positive Control
HEK-293 cell lysate, HepG2 cell lysate, Raji cell lysate, HCT 116 cell lysate, HeLa cell lysate, HeLa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE44-67
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 68 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SYVN1
SYNONYMS
1200010C09Rik antibody;DER3 antibody;E3 ubiquitin-protein ligase synoviolin antibody;HMG coA reductase degradation 1 homolog antibody;HRD1 antibody;KIAA1810 antibody;MGC40372 antibody;OTTHUMP00000230429 antibody;OTTHUMP00000230430 antibody;OTTHUMP00000230431 antibody;OTTHUMP00000230432 antibody;Synovial apoptosis inhibitor 1 antibody;Synovial apoptosis inhibitor 1, synoviolin antibody;Synoviolin 1 antibody;Synoviolin 1 isoform b antibody;SYNOVIOLIN antibody;SYVN1 antibody;SYVN1_HUMAN antibody
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane.
FUNCTION
E3 ubiquitin-protein ligase synoviolin is an enzyme that in humans is encoded by the SYVN1 gene. This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. This gene and the mitochondrial ribosomal protein L49 gene use in their respective 3' UTRs some of the same genomic sequence. Sequence analysis identified two transcript variants that encode different isoforms.
CITATIONS
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Wu Sizhi,et al
Discovery of Momordin Ic that selectively reduces PD-L1 expression in multiple myeloma cells by recruiting SYVN1
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WB
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IF
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Human
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Mouse
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Citation
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