Syntaxin 3 Recombinant Rabbit Monoclonal Antibody [JE40-96] (ET7109-19)
Catalog# ET7109-19
Rabbit Monoclonal to Syntaxin 3
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WB
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IHC-P
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Human
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Mouse
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Rat
Western blot analysis of Syntaxin 3 on different lysates with Rabbit anti-Syntaxin 3 antibody (ET7109-19) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Syntaxin 3 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-19) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Syntaxin 3 Recombinant Rabbit Monoclonal Antibody [JE40-96] (ET7109-19)
Immunogen
Recombinant protein within human syntaxin 3 aa 92-218 / 289.
Host
Rabbit
Positive Control
293T cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse kidney tissue, mouse retina tissue, rat kidney tissue, rat retina tissue, human prostate cancer tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE40-96
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:2,000-1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Syntaxin 3
SYNONYMS
FLJ30906 antibody;Stx3 antibody;STX3_HUMAN antibody;STX3A antibody;Syntaxin 3A antibody;Syntaxin-3 antibody
SEQUENCE SIMILARITIES
Belongs to the syntaxin family.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Correct vesicular transport is essential to the survival of eukaryotic cells. This process is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). This complex then recruits soluble NSF attachment proteins (SNAPs) and N-ethylmaleimide-sensitive factor (NSF) to form the highly stable SNAP receptor (SNARE) complex. The formation of a SNARE complex pulls the vesicle and target membrane together and may provide the energy to drive fusion of the lipid bilayers. Syntaxins, a family of proteins involved in the fusion of synaptic vesicles with the plasma membrane, display broad tissue distribution and contain carboxy-terminal hydrophobic domains that direct themselves to their respective intracellular compartments. Syntaxin 3 localizes to the apical plasma membrane and is involved in membrane fusion of apical trafficking pathways. Syntaxin 3 is a key factor in the growth of neurites, and it also functions as a direct target for arachidonic acid. Human Syntaxin 3 has two forms: Syntaxin 3A and 3B, while the mouse version has four forms: 3A, 3B, 3C, and 3D.
CITATIONS
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Zhang, X., Deng, D., Cui, D., Liu, Y., He, S., Zhang, H., Xie, Y., Yu, X., Yang, S., Chen, Y., & Su, Z.
Cholesterol Sulfate Exerts Protective Effect on Pancreatic β-Cells by Regulating β-Cell Mass and Insulin Secretion
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WB
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Mouse
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Citation
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