Synaptopodin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH10-45] (HA751350)
Catalog# HA751350
Rabbit Monoclonal to Synaptopodin
Tested for
-
WB
-
IHC-Fr
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Synaptopodin on different lysates with Rabbit anti-Synaptopodin antibody (HA751350) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse liver tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lane 4: Rat liver tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751350) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Synaptopodin on different lysates with Rabbit anti-Synaptopodin antibody (HA751350) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse liver tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lane 4: Rat liver tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751350) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.