PRODUCT CODE: 0407-2

Synaptophysin Rabbit Polyclonal Antibody (0407-2)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin antibody at 1/1,000 dilution.
  • Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin antibody at 1/1,000 dilution.
  • ICC staining Synaptophysin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SHG-44 cells with Synaptophysin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Synaptophysin Rabbit Polyclonal Antibody (0407-2)

Immunogen

Synthetic peptide (klh-coupled) within human synaptophysin 220-280 aa.

Host

Rabbit

Positive Control

Human brain tissue, mouse brain tissue, N2A, rat pancreas tissue, SHG-44.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

34kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:200-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Synaptophysin

SYNONYMS

Major synaptic vesicle protein p38 antibody; MRX96 antibody; MRXSYP antibody; Syn p38 antibody; Synaptophysin antibody; Syp antibody; SYPH antibody; SYPH_HUMAN antibody; SypI antibody

SEQUENCE SIMILARITIES

Belongs to the synaptophysin/synaptobrevin family.

TISSUE SPECIFICITY

Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasmic vesicle. Membrane. Synapse. Synaptosome.

FUNCTION

Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.