Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Synaptophysin Rabbit Polyclonal Antibody (0407-2)
Immunogen
Synthetic peptide (klh-coupled) within human synaptophysin 220-280 aa.
Host
Rabbit
Positive Control
Human brain tissue, mouse brain tissue, N2A, rat pancreas tissue, SHG-44.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
34kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:200-1:1,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Synaptophysin
SYNONYMS
Major synaptic vesicle protein p38 antibody; MRX96 antibody; MRXSYP antibody; Syn p38 antibody; Synaptophysin antibody; Syp antibody; SYPH antibody; SYPH_HUMAN antibody; SypI antibody
SEQUENCE SIMILARITIES
Belongs to the synaptophysin/synaptobrevin family.
TISSUE SPECIFICITY
Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
SUBCELLULAR LOCATION
Cytoplasmic vesicle. Membrane. Synapse. Synaptosome.
FUNCTION
Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.