Synaptophysin Recombinant Rabbit Monoclonal Antibody [SJ26-85] (ET1606-56)
Catalog# ET1606-56
Rabbit Monoclonal to Synaptophysin
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: Not required
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Synaptophysin Recombinant Rabbit Monoclonal Antibody [SJ26-85] (ET1606-56)
Immunogen
Synthetic peptide within human synaptophysin aa 224 – 313 (cytoplasmic).
Host
Rabbit
Positive Control
SH-SY5Y cell lysate, PC-12 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, atypical carcinoid tissue, human medullary thyroid carcinoma tissue, human pancreas tissue, human small intestine tissue, mouse cerebellum tissue, mouse pancreas tissue, rat cerebellum tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SJ26-85
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:10,000
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IHC-P
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1:2,000
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IHC-Fr
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1:1,000
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IF-Tissue
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 34 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Synaptophysin
SYNONYMS
Major synaptic vesicle protein p38 antibody;MRX96 antibody;MRXSYP antibody;Syn p38 antibody;Synaptophysin antibody;Syp antibody;SYPH antibody;SYPH_HUMAN antibody;SypI antibody
SEQUENCE SIMILARITIES
Belongs to the synaptophysin/synaptobrevin family.
TISSUE SPECIFICITY
Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
SUBCELLULAR LOCATION
Cytoplasmic, Cell junction, synapse, synaptosome.
FUNCTION
Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis. In addition, the tail domain of brain Myosin V also forms a stable complex with synaptobrevin II and SYP, and this complex is disassembled upon the depolarization-induced entry of Ca2+ into intact nerve endings.
CITATIONS
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Wang Xiaohong,et al
Deciphering the Therapeutic Potential of SheXiangXinTongNing: Interplay between Gut Microbiota and Brain Metabolomics in a CUMS Mice Model, with a Focus on Tryptophan Metabolism
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IF
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WB
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Mouse
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Citation
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Mitochondrial protein prohibitin promotes learning memory recovery in mice following intracerebral hemorrhage via CAMKII/CRMP signaling pathway
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IF
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Mouse
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Citation
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