PRODUCT CODE: ET7108-92

SUN2 Recombinant Rabbit Monoclonal Antibody [JG39-52] (ET7108-92)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of SUN2 on rat kidney tissue lysate using anti-SUN2 antibody at 1/2,000 dilution.
  • Western blot analysis of SUN2 on rat kidney tissue lysate using anti-SUN2 antibody at 1/2,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
  • ICC staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SK-Br-3 cells with SUN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SUN2 on rat kidney tissue lysate using anti-SUN2 antibody at 1/2,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SUN2 Recombinant Rabbit Monoclonal Antibody [JG39-52] (ET7108-92)

Immunogen

Recombinant protein within human sun2 aa 500-700.

Host

Rabbit

Positive Control

Rat kidney tissue lysate, rat epididymis tissue, human tonsil tissue, human colon tissue, human prostate cancer tissue, mouse liver tissue, SK-Br-3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG39-52

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

80 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SUN2

SYNONYMS

FRIGG antibody; KIAA0668 antibody; nuclear envelope protein antibody; Protein unc-84 homolog B antibody; Rab5 interacting protein antibody; RAB5IP antibody; Sad1 and UNC84 domain containing 2 antibody; Sad1 unc-84 domain protein 2 antibody; Sad1 unc84 domain protein antibody; Sad1/unc-84 protein-like 2 antibody; Sad1/unc84 protein-like antibody; SUN domain-containing protein 2 antibody; UNC 84B antibody; unc84 homolog B (C. elegans) antibody; unc84 homolog B antibody; unc84, C. elegans, homolog of, B antibody; UNC84B antibody

TISSUE SPECIFICITY

Widely expressed. Highly expressed in heart, lung and muscle. Weakly expressed in fetal heart. Slightly overexpressed in some heart tissues form patients with congenital heart defects.

POST-TRANSLATIONAL MODIFICATION

The disulfid bond with SYNE2 is required for stability of the SUN2:SYNE2/KASH2 LINC complex under tensile forces though not required for the interaction. The disulfid bond is proposed to be conserved in LINC complexes involved in force transmission.

SUBCELLULAR LOCATION

Endosome. Nucleus inner membrane.

FUNCTION

SUN2 (sad1/unc-84 protein-like 2), also known as UNC84B (unc-84 homolog B), FRIGG, KIAA0668 or RAB5IP, is a 717 amino acid single-pass membrane protein that contains one SUN domain and localizes to the membrane of both the nucleus and the endosome. Widely expressed in a variety of tissues, including lung, muscle and heart, SUN2 interacts with Rab 5A and may play a role in homotypic endosome fusion. The gene encoding SUN2 maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia. Additionally, translocations between chromosomes 9 and 22 may lead to the formation of the Philadelphia Chromosome and the subsequent production of the novel fusion protein BCR-Abl, a potent cell proliferation activator found in several types of leukemias.