PRODUCT CODE: ET1701-8

SUMO4 Recombinant Rabbit Monoclonal Antibody [JJ085-01] (ET1701-8)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SUMO4 on different lysates using anti-SUMO4 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: 293T           <br />
Lane 2: Jurkat
  • Western blot analysis of SUMO4 on different lysates using anti-SUMO4 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: 293T           <br />
Lane 2: Jurkat
  • ICC staining SUMO4 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUMO4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUMO4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUMO4 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of 293 cells with SUMO4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of SUMO4 on different lysates using anti-SUMO4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Jurkat

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SUMO4 Recombinant Rabbit Monoclonal Antibody [JJ085-01] (ET1701-8)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, MCF-7, 293, HepG2, Jurkat, RH-35.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ085-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

SUMO4

SYNONYMS

dJ281H8.4 antibody; IDDM5 antibody; Small ubiquitin like modifier 4 protein antibody; Small ubiquitin-like protein 4 antibody; Small ubiquitin-related modifier 4 antibody; SMT3 suppressor of mif two 3 homolog 2 antibody; SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae) antibody; SMT3H4 antibody; SUMO 4 antibody; SUMO-4 antibody; SUMO4 antibody; SUMO4_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the ubiquitin family. SUMO subfamily.

TISSUE SPECIFICITY

Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen.

POST-TRANSLATIONAL MODIFICATION

In contrast to SUMO1, SUMO2 and SUMO3, seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates.

FUNCTION

The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2, SUMO-3 and SUMO-4, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Ubiquitin and SUMO proteins utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2, SUMO-3 and SUMO-4 proteins localize to the nucleus. In contrast to the other SUMO proteins, SUMO-4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90, which may impair processing to mature form and conjugation to substrates. It is suggested that defects in the gene that encodes for the SUMO-4 protein may be involved in the pathogenesis of type I diabetes.